Has anybody on the list a URL to a web resource which details the techniques
used by Hansen (or a modern equivalent) to isolate single cells of yeast.

Lest you think me an idle Brit I have spent much time Googling with the string
<"emil hansen" techniques yeast "serial dilution"> and many variations, adding
in "laboratory" "microbiology" and "beer".

TIA

John

Hi John;

Yes, I've diluted a culture for microscopy. I did it out of curiosity and
to see how well I could ID and sort out the critters involved--a 1% aqueous
solution of methylene blue sure helps...

What is it that you'd like to know?

Perhaps this s/b an off-line discussion...?


Dusty
--
Remove STORE to reply
"Wcsjohn" > wrote in message
...
> Has anybody on the list a URL to a web resource which details the
techniques
> used by Hansen (or a modern equivalent) to isolate single cells of yeast.
>
> Lest you think me an idle Brit I have spent much time Googling with the
string
> <"emil hansen" techniques yeast "serial dilution"> and many variations,
adding
> in "laboratory" "microbiology" and "beer".
>
> TIA
>
> John
>
>
>
>
>
>

>
>Hi John;
>
>Yes, I've diluted a culture for microscopy. I did it out of curiosity and
>to see how well I could ID and sort out the critters involved--a 1% aqueous
>solution of methylene blue sure helps...
>
>What is it that you'd like to know?
>
>Perhaps this s/b an off-line discussion...?
>
>
>Dusty

I think you're right - a little off-topic - I'll email you.

John

Wcsjohn wrote:

> I think you're right - a little off-topic - I'll email you.


I think it's on topic. Feel free to discuss it in this group,
at your option of course.

I've had my nose in several microbiology books trying to find
the best way to do this myself, so I'd be interested.

--
Reg email: RegForte (at) (that free MS email service) (dot) com

"Dusty" > wrote in message
...
> Hi John;
>
> Yes, I've diluted a culture for microscopy. I did it out of curiosity and
> to see how well I could ID and sort out the critters involved--a 1%
aqueous
> solution of methylene blue sure helps...
>
> What is it that you'd like to know?
>
> Perhaps this s/b an off-line discussion...?
>
>
> Dusty
> --
>
I'd be interested too as I use a high-powered biological microscope (Leitz
Orthoplan) to study fossil fungi, spores, pollen and microplankton. I've
looked at some yeast cells but I don't see how I could speciate them at
normal magnifications.
Graham

gdotdolby((at))shawdotca

>>
>I'd be interested too as I use a high-powered biological microscope (Leitz
>Orthoplan) to study fossil fungi, spores, pollen and microplankton. I've
>looked at some yeast cells but I don't see how I could speciate them at
>normal magnifications.
>Graham
>
>gdotdolby((at))shawdotca
>
Unless I've misread, Hansen pr

Sorry!, cheap fingers and AOL si palying up, keep losing connrction.

Later

John

"Wcsjohn" > wrote in message
...
> Sorry!, cheap fingers
Of Scotch?<g>

>and AOL si palying up,

Ironically, John, the science of studying fungal and plant spores and
pollen etc., is palynology (correct spelling, even after a couple of glasses
of claret).
Graham

"Wcsjohn" > wrote in message =
...

> ... I have spent much time Googling with the string "emil hansen"=20
> techniques yeast "serial dilution" and many variations, adding
> in "laboratory" "microbiology" and "beer".

Google "streak-plate". You may find, for instance,
http://www.austin.cc.tx.us/microbugz/18streakiso.html

Another such technique is "pour-plate". That is consistent
with "serial dilution" but is more appropriate to counting
microorganisms than to isolating single colonies.

Those techniques are appropriate to microbiological labs,
but hardly to kitchens.

"Wcsjohn" > wrote in message =
...

> ... a little off-topic ...

Bread and beer can be made with very little knowledge of=20
microbiology. But posters to this thread may be interested in
http://aem.asm.org/cgi/content/full/64/7/2616
and the references given there.

Be sure you understand all that before you attempt to bake
another loaf of bread.

>
>> ... I have spent much time Googling with the string "emil hansen"=20
>> techniques yeast "serial dilution" and many variations, adding
>> in "laboratory" "microbiology" and "beer".
>
>Google "streak-plate". You may find, for instance,
>http://www.austin.cc.tx.us/microbugz/18streakiso.html
>
>Another such technique is "pour-plate". That is consistent
>with "serial dilution" but is more appropriate to counting
>microorganisms than to isolating single colonies.
>
>Those techniques are appropriate to microbiological labs,
>but hardly to kitchens.
>
<snip>

I agree, I am just following my curiosity on this topic.

Thank you for the search terms - If you don't know what it's called you can't
Google it.

John

Try the n.g. sci.bio.food-science

or

sci.bio.microbiology


When I recall my home beer making, I seem to recall Hansen used a
"polarized" microscope. But I'm recalling this from memory of over 15
years ago.